Spoluautoři KUBÁSEK Jiří, SCHWARZ Marek, LIPOV Jan, VOJTĚCH Dalibor, RUML Tomáš

Magnesium alloys are potential biodegradable materials for biomedical load-bearing applications. Magnesium itself is regarded as biocompatible, biodegradable and osteoconductive material with similar elastic modulus to that of human bone; however, its strength is unsatisfactory for orthopaedic applications. Therefore, addition of alloying elements is necessary. Biocompatibility of these alloying elements and of prepared alloys is evaluated using in vitro assays. To select the most suitable alloying elements, we tested in vitro toxicity of chloride salts of variety of elements on human cell line derived from osteosarcoma (U-2 OS). Cytotoxicity was determined using colorimetric WST-1 test. We have investigated the complexity of the system, which is affected by many factors, i.e. by concentration of FBS in medium, by putative precipitation of cations with phosphates in the growth medium or by lack of transporters for non-biogenic elements. We have tried to elucidate all these factors in order to simulate the effect of corrosion products by salts of alloying elements.